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	<title>Comments on: Standalone BLAST with Ruby (windows)</title>
	<atom:link href="http://biorelated.com/2007/10/03/standalone-blast-with-ruby-part-1/feed/" rel="self" type="application/rss+xml" />
	<link>http://biorelated.com/2007/10/03/standalone-blast-with-ruby-part-1/</link>
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		<title>By: yannick</title>
		<link>http://biorelated.com/2007/10/03/standalone-blast-with-ruby-part-1/#comment-202</link>
		<dc:creator><![CDATA[yannick]]></dc:creator>
		<pubDate>Wed, 22 Jun 2011 12:35:54 +0000</pubDate>
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		<description><![CDATA[You can also use ruby to set up a &lt;a href=&quot;http://www.sequenceserver.com&quot; rel=&quot;nofollow&quot;&gt;local BLAST server&lt;/a&gt;, much easier than would be using NCBI&#039;s wwwblast.
Cheers!]]></description>
		<content:encoded><![CDATA[<p>You can also use ruby to set up a <a href="http://www.sequenceserver.com" rel="nofollow">local BLAST server</a>, much easier than would be using NCBI&#8217;s wwwblast.<br />
Cheers!</p>
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		<title>By: jan.</title>
		<link>http://biorelated.com/2007/10/03/standalone-blast-with-ruby-part-1/#comment-62</link>
		<dc:creator><![CDATA[jan.]]></dc:creator>
		<pubDate>Thu, 24 Jan 2008 15:59:43 +0000</pubDate>
		<guid isPermaLink="false">http://biorelated.wordpress.com/2007/10/03/standalone-blast-with-ruby-part-1/#comment-62</guid>
		<description><![CDATA[You can also use bioruby to run BLAST searches against local databases. Here&#039;s a sample script:
&lt;code&gt;
  #!/usr/bin/ruby
  require &#039;bio&#039;
  
  factory = Bio::Blast.local(&#039;blastn&#039;, &#039;/path/to/BLAST/database&#039;)
  ff = Bio::FlatFile.open(Bio::FastaFormat, &#039;input_file.fa&#039;)
  ff.each do &#124;entry&#124;
    $stderr.puts &quot;Searching...&quot; + entry.definition
    report = factory.query(entry)
    report.each do &#124;hit&#124;
      hit.each do &#124;hsp&#124;
        puts hsp.query_from
      end
    end
  end
&lt;/code&gt;]]></description>
		<content:encoded><![CDATA[<p>You can also use bioruby to run BLAST searches against local databases. Here&#8217;s a sample script:<br />
<code><br />
  #!/usr/bin/ruby<br />
  require 'bio'</p>
<p>  factory = Bio::Blast.local('blastn', '/path/to/BLAST/database')<br />
  ff = Bio::FlatFile.open(Bio::FastaFormat, 'input_file.fa')<br />
  ff.each do |entry|<br />
    $stderr.puts "Searching..." + entry.definition<br />
    report = factory.query(entry)<br />
    report.each do |hit|<br />
      hit.each do |hsp|<br />
        puts hsp.query_from<br />
      end<br />
    end<br />
  end<br />
</code></p>
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